reference mouse anti bmp9 antibody  (R&D Systems)

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: An angiopoietin-2 vaccine improves arteriovenous malformation pathology in hereditary hemorrhagic telangiectasia mice

doi: 10.1016/j.bvth.2026.100155

Figure Lengend Snippet: Immunogenicity of the ANG2-P3:CRM197 vaccine in C57BL/6 mice. (A) Schematic diagram of the vaccination schedule and generation of the BMP9/10ib model (created with biorender.com ). (B) Protein sequence alignment of the C-terminal end of human ANG2 (hANG2), mouse ANG2 (mANG2), human ANG1 (hANG1), and mouse ANG1 (mANG1), along with the peptide sequences of ANG2-P3 and ANG1-P3. (C-D) Serum antibody titers against ANG2-P3 (C) and ANG1-P3 (D) in ANG2-P3:CRM197-vaccinated females (Vac-1 to Vac-5) and controls (injected with saline [Sal-1 and Sal-2] or CRM197-only [CRM-1 to CRM-5]). The vaccinated females with the highest anti-ANG2-P3 titers were identified as “best responders” (marked with a red box). OD, optical density.

Article Snippet: After 3 additional washes with PBST, serial dilutions of individual mouse serum samples were prepared, along with a reference mouse anti-BMP9 antibody (no. MAB3209; R&D Systems) and a reference mouse anti-BMP10 antibody (no. MAB2926; R&D Systems), both diluted in 1% BSA in PBS.

Techniques: Immunopeptidomics, Sequencing, Injection, Saline

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: An angiopoietin-2 vaccine improves arteriovenous malformation pathology in hereditary hemorrhagic telangiectasia mice

doi: 10.1016/j.bvth.2026.100155

Figure Lengend Snippet: ANG2-P3:CRM197 vaccine reduces AVM pathology in BMP9/10ib neonates. (A) Serum antibody titers against ANG2-P3 in pups and their corresponding dams vaccinated with ANG2-P3:CRM197 (Vac-1 to Vac-3) or injected with saline (Saline). Vac-1, Vac-2, and Vac-3 pups: n = 7; Saline pups: n = 2. Dams: n = 1. (B) Representative immunofluorescence staining with isolectin B4 (IB4; green) and of SMA (red) in P6 retinas of pups treated with PBS or BMP9/10ib from a dam vaccinated with ANG2-P3:CRM197 or injected with saline. Scale bar: 1.5 mm. (C-D ) AVM count per retina (C), and retinal AVM surface area (D) in BMP9/10ib pups from dams vaccinated with ANG2-P3:CRM197 or injected with saline (Saline). BMP9/10ib + saline: n = 24; BMP9/10ib + ANG2-P3:CRM197: n = 37 (C). BMP9/10ib + saline: n = 24; BMP9/10ib + ANG2-P3:CRM197: n = 34 (D). (E) Spearman rank correlation matrix of the indicated variables (n = 15). (F-H) Retinal artery diameter (F), retinal vein diameter (G), and SMA coverage area (H) in BMP9/10ib pups from dams vaccinated with ANG2-P3:CRM197 or injected with saline (Saline). Data are shown as mean ± standard error of the mean (SEM); unpaired t test with Welch correction for panel C, Mann-Whitney test for panel D, and 1-way analysis of variance with Tukey multiple comparisons test for panels F-H. PBS + saline: n = 15; BMP9/10ib + saline: n = 24; BMP9/10ib + ANG2-P3:CRM197: n = 37 (F,G). PBS + saline: n = 13; BMP9/10ib + saline: n = 21; BMP9/10ib + ANG2-P3:CRM197: n = 29 (H). ∗ P < .05; ∗∗∗ P ≤ .001; ∗∗∗∗ P < .0001. (I) Immunoassay data measuring ANG2 levels in plasma from patients with HHT and healthy controls. Data are shown as mean ± SEM; unpaired t test with Welch correction. Healthy: n = 36; HHT: n = 36. ∗∗ P ≤ .01. a, artery; ns, not significant; v, vein.

Article Snippet: After 3 additional washes with PBST, serial dilutions of individual mouse serum samples were prepared, along with a reference mouse anti-BMP9 antibody (no. MAB3209; R&D Systems) and a reference mouse anti-BMP10 antibody (no. MAB2926; R&D Systems), both diluted in 1% BSA in PBS.

Techniques: Injection, Saline, Immunofluorescence, Staining, MANN-WHITNEY, Clinical Proteomics

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: ( A–D ) ELISAs were performed to measure IgG2a ( A , C ) and anti-BMP9 Ab ( B , D ) levels in the serum of P6 neonates treated at P3 with vehicle (PBS), isotype control IgGs (IgG2a/2b), or anti-BMP9/10 Abs. Neonates were treated during lactation either from dams injected i.p. with ( A , B ), or by direct i.p. injections of ( C , D ), the different Abs or vehicle. Data represent mean ± s.e.m. (n = 5–7 pups per group from 2 dams); **** P < 0.0001, ANOVA.

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: Control, Injection

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: ( A – C ”) Representative images of fluorescent isolectin B4-stained retinas either from P6 neonates fed for 3 days by dams injected on P3 with PBS ( A ), control IgG2a/b Abs ( B ), or BMP9/10 blocking Abs ( C – C ”). ( C’ , C” ) are higher magnification images of the relevant boxed areas in C. a, artery; v, vein; scale bars, 500 μm. ( D–L ) Higher magnification showing retinal vasculature fields between an artery and a vein (Plexus, D–F ), or at the front of an artery ( G–I ) or a vein ( J–L ) from neonates treated as in ( A–C ). Scale bars, 100 μm. ( M–O ) Scatter plots showing the vascular density on retinal ‘petals’ at the plexus ( M ), artery front ( N ), or vein front ( O ). Data represent mean ± s.e.m. (n = 6 pups per group from 2 dams); **** P < 0.0001, ANOVA and Kruskal-Wallis test. Arrows indicate AVMs, defined as direct shunts between an artery and a vein.

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: Staining, Injection, Control, Blocking Assay

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: ( A,B ) Representative images of blue latex-perfused retinal vasculature of P6 neonates fed for 3 days by dams injected on P3 with control IgG2a/b Abs ( A ) or BMP9/10 blocking Abs ( B ). Arrows in B indicate AVMs. Scale bars, 500 μm. ( C ) Scheme depicting the method employed for the quantification of the number of latex dye-positive vessels. ( D ) Histogram showing the total number of vascular crosses. ( E ) Histogram showing the number of vascular crosses per concentric circle on circles 1 to 3. Data represent mean ± s.e.m. (n = 5–7 pups per group from 2 dams); **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05; ANOVA and Kruskal-Wallis test ( D , E ).

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: Injection, Control, Blocking Assay

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: ( A ) RNA-Seq heat map displaying differently expressed genes in mouse whole retinas following transmammary transfer of anti-BMP9/10 or control IgG2a/2b Abs (n = 6 pups per group from 1 dam). ( B ) HUVECs were treated or not (Ctrl) with ALK1-Fc (1 μg/mL, 24 h). Cell extracts were then analyzed by WB using Abs directed against the indicated proteins. ( C ) Densitometric analyses and quantification of phospho-Smad1/5/8, ID1, and ANG2 relative levels in three independent experiments as in ( B ). ( D ) ECs isolated from retinas of pups fed for 3 days by dams injected on P3 with control IgG2a/b Abs (Ctrl) or BMP9/10 blocking Abs (anti-BMP9/10) were analyzed for Id1 and Angpt2 mRNA levels by RT-qPCR. The results are expressed as relative levels of the control condition (n = 3 determinations, n = 6 pups per group from 1 dam). Data in ( C , D ) are mean ± s.e.m.; *** P < 0.001, ** P < 0.01, * P < 0.05, unpaired Student’s t-test.

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: RNA Sequencing, Control, Isolation, Injection, Blocking Assay, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10

doi: 10.1038/srep37366

Figure Lengend Snippet: Top 25 retinal genes sorted by P -value, following transmammary-delivered immunoblocking of BMP9 and BMP10.

Article Snippet: After washing 3 times with PBST, serial dilutions of individual mouse serum samples and reference mouse anti-BMP9 Ab (MAB3209, R&D Systems) (diluted in 1% BSA PBS) were prepared and 100 μL/well were incubated for 2 h at RT.

Techniques: Sterility, Binding Assay, Sequencing